Cells, Free Full-Text

We present a multidisciplinary approach for predicting how sperm cells with various morphologies swim in three-dimensions (3D), from milliseconds to much longer time scales at spatial resolutions of less than half a micron. We created the sperm 3D geometry and built a numerical mechanical model using the experimentally acquired dynamic 3D refractive-index profiles of sperm cells swimming in vitro as imaged by high-resolution optical diffraction tomography. By controlling parameters in the model, such as the size and shape of the sperm head and tail, we can then predict how different sperm cells, normal or abnormal, would swim in 3D, in the short or long term. We quantified various 3D structural factor effects on the sperm long-term motility. We found that some abnormal sperm cells swim faster than normal sperm cells, in contrast to the commonly used sperm selection assumption during in vitro fertilization (IVF), according to which sperm cells should mainly be chosen based on their progressive motion. We thus establish a new tool for sperm analysis and male-infertility diagnosis, as well as sperm selection criteria for fertility treatments.

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Oxidative stress. From normal cells, to oxidative stress and aggressive free radicals,to cell death.Educational and study content for biology students Stock Vector Image & Art - Alamy

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Frontiers Mouse Microbiota Models: Comparing Germ-Free Mice and Antibiotics Treatment as Tools for Modifying Gut Bacteria

Plasma cell-free DNA (cfDNA) as a predictive and prognostic marker in patients with metastatic breast cancer, Breast Cancer Research

Cells, Free Full-Text

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Nucleic acid biomarkers of immune response and cell and tissue damage in children with COVID-19 and MIS-C - ScienceDirect

Anti-HIV-1 Activity of Crocodylus mindorensis Philippine Crocodile Serum in Cell-free and Cell-associated Virus Interactions to Human Peripheral Blood Mononuclear Cells